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Phusion DNA Polymerase possesses 5´→ 3´ polymerase activity, 3´→ 5´ exonuclease activity and will generate blunt-ended products. Phusion High-Fidelity PCR Master Mix with HF Buffer is a 2X master mix consisting of Phusion DNA Polymerase, deoxynucleotides and reaction buffer that has been optimized and includes MgCl 2.
Jul 07, 2021 · I'm thinking between Invitrogen's Platinum Taq Hotstart, Kapa Biosystem's HI-FI Hotstart and NEB's Phusion high-fidelity. I'm not sure which will yield the best amount of product and specificity
PCR Protocol for Phusion ® High-Fidelity DNA Polymerase (M0530) Protocols.io also provides an interactive version of this protocol where you can discover and share optimizations with the research community. Overview. The following guidelines are provided to ensure successful PCR using Phusion ® DNA Polymerase.These guidelines cover routine PCR.
Phusion® Technology. Phusion DNA Polymerase brings together a novel Pyrococcus-like enzyme with a processivity-enhancing domain and generates PCR products with accuracy and speed previously unattainable with a single enzyme, even on your most difficult templates. Additionally, Phusion DNA Polymerase is capable of amplifying long templates. In the quality control tests of Phusion DNA
Phusion. GC Buffer Pack. New England Biolabs supplies a unique 5X reaction buffer and supplemental reagents for use with Thermo Scientific® Phusion High-Fidelity DNA Polymerase and associated products, all of which are manufactured and quality controlled at New England Biolabs.
Phusion® High-Fidelity PCR Kit. Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at [email protected] or x 8.
Jul 07, 2021 · I'm thinking between Invitrogen's Platinum Taq Hotstart, Kapa Biosystem's HI-FI Hotstart and NEB's Phusion high-fidelity. I'm not sure which will yield the best amount of product and specificity
Phusion Hot Start Flex DNA Polymerase is supplied with standard 5X Phusion HF Buffer, as well as 5X Phusion GC Buffer, which can be used for complex or GC-rich templates. Each of these buffers contains MgCl 2 (1.5 mM at the final [1X] reaction concentration). Reactions can also be optimized using the provided DMSO or MgCl 2 solutions.
Aug 18, 2017 · Proof (800bp) Tried homemade buffer #1 vs storebought NEB 5X HF buffer with homemade phusion, PCR for mTAG-BFP2 from ~25 ng plasmid. Overall it worked, if I’m being picky there seems to be a bit more smearing in the homemade buffer, however the amount of desired product is the same between the two buffers.
Phusion® Technology. Phusion DNA Polymerase brings together a novel Pyrococcus-like enzyme with a processivity-enhancing domain and generates PCR products with accuracy and speed previously unattainable with a single enzyme, even on your most difficult templates. Additionally, Phusion DNA Polymerase is capable of amplifying long templates. In the quality control tests of Phusion DNA
Description. This package includes a Phusion Site-Directed Mutagenesis Kit and DH10B Competent Cells that are optimized for use with the kit. The Thermo Scientific Phusion Site-Directed Mutagenesis Kit is a versatile and efficient tool for introducing point mutations, insertions, or
Phusion High-Fidelity PCR Master Mix with GC Buffer offers robust performance and can be used for all PCR applications. It is a 2X master mix consisting of Phusion DNA Polymerase, deoxynucleotides and reaction buffer that has been optimized and includes MgCl 2. All that is required is the addition of template, primers and water.
For any questions, please use our Phusion Request for Technical Support or call NEB Technical Support at . Phusion® is a registered trademark and property of Thermo Fisher Scientific. Fidelity assays were performed using a lacI-based method modified from Frey & Suppman, 1995.
Phusion® GC Buffer Pack. New England Biolabs supplies a unique 5X reaction buffer and supplemental reagents for use with Thermo Scientific® Phusion High-Fidelity DNA Polymerase and associated products, all of which are manufactured and quality controlled at New England Biolabs.
Phusion Hot Start Flex DNA Polymerase is available as standalone enzyme or in a master mix format and enables high specificity amplification of a broad range of templates. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. NEB offers a guidelines for choosing the correct DNA polymerase for
Phusion® DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific. Notice to purchaser Limited license. The purchase price of this product includes a limited, non-transferable license under U.S. and foreign patents owned by BIO-RAD Laboratories, Inc., to
NEB Tm Calculator. Product Group. Q5 Q5 Hot Start Q5U Hot Start OneTaq OneTaq Hot Start Hot Start Taq Taq DNA Polymerase LongAmp Taq LongAmp Hot Start Taq Hemo KlenTaq Vent Deep Vent Master Mix Phusion Phusion Hot Start Flex EpiMark Hot Start. Polymerase/Kit. Q5 High-Fidelity DNA Polymerase Q5 High-Fidelity 2X Master Mix. Primer Concentration
2 days ago · forward and reverse primers from a 100 μM stock. Amplification used Phusion High Fidelity polymerase (NEB) and the following cycling protocol 98°C for 30 seconds, (98°C for 10 seconds, 58°C for 30 seconds, 72°C for 60 seconds) × 6, 72°C for 5 minutes. PCR products were purified (Zymo DNA Clean & Concentrator kit) and then combined.
Jul 05, 2021 · Starting with 250 ng of total DNA, UpTag and DnTag barcodes were separately amplified with Phusion ® High-Fidelity DNA polymerase (NEB, UK) using custom-designed primers at a concentration of 100 nM each in a total volume of 50 µL, with 6 cycles of 10 seconds at 98°C, 30 seconds at 60°C and 30 seconds at 72°C.
Phusion DNA Polymerase is supplied with standard 5X Phusion HF Buffer, as well as 5X Phusion GC Buffer, which can be used for complex or GC-rich templates. Each of these buffers contains MgCl 2 (1.5 mM at the final [1X] reaction concentration). Reactions
Phusion® Technology. Phusion DNA Polymerase brings together a novel Pyrococcus-like enzyme with a processivity-enhancing domain and generates PCR products with accuracy and speed previously unattainable with a single enzyme, even on your most difficult templates. Additionally, Phusion DNA Polymerase is capable of amplifying long templates. In the quality control tests of Phusion DNA
Phusion Hot Start Flex DNA Polymerase is supplied with standard 5X Phusion HF Buffer, as well as 5X Phusion GC Buffer, which can be used for complex or GC-rich templates. Each of these buffers contains MgCl 2 (1.5 mM at the final [1X] reaction concentration). Reactions can also be optimized using the provided DMSO or MgCl 2 solutions.
Jul 05, 2021 · Starting with 250 ng of total DNA, UpTag and DnTag barcodes were separately amplified with Phusion ® High-Fidelity DNA polymerase (NEB, UK) using custom-designed primers at a concentration of 100 nM each in a total volume of 50 µL, with 6 cycles of 10 seconds at 98°C, 30 seconds at 60°C and 30 seconds at 72°C.
The Phusion™ Site-Directed Mutagenesis Kit contains reagents for a total of 20 mutagenesis reactions (including control reactions), and control plasmid and primers for 10 reactions. Component Amount Storage Phusion™ Hot Start II DNA Polymerase, 2 U/μL 10 µL −20°C 5X Phusion™ HF Buffer 1.5 mL dNTP Mix 10 mM each 20 µL FastDigest
Includes. Phusion High-Fidelity DNA Polymerase is supplied with 5x Phusion HF Buffer, 5x Phusion GC Buffer, DMSO and 50 mM MgCl2 solution. Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1x concentration. Quantity. 500U (2U/µL) Concentration. 2 U/µL.
Web pricing is applicable only to orders placed online at neb.ca. Product Introduction. Create optimal reaction conditions for high fidelity amplification. The default buffer for use with Phusion PHUSION® is a registered trademark and property of Thermo Fisher Scientific.
Phusion. From Synthetic construct (aliases Phusion DNA Polymerase) Phusion is an enzyme of unknown family from Synthetic construct . test.
Phusion Green Hot Start II High-Fidelity PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phusion Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl 2. The buffer also includes a density reagent and two tracking dyes for direct loading of PCR
To estimate the activity, create a dilution series of purified polymerase in water ranging from 1 200 to 1 10, and compare to NEB Phusion or a similar polymerase. NEB stock is viscous for an accurate comparison to the purified polymerase, ensure you are pipetting sufficient volumes to maintain accuracy.
Phusion High-Fidelity PCR Master Mix with GC Buffer offers robust performance and can be used for all PCR applications. It is a 2X master mix consisting of Phusion DNA Polymerase, deoxynucleotides and reaction buffer that has been optimized and includes MgCl 2. All that is required is the addition of template, primers and water.
Phusion High-Fidelity PCR Master Mix with GC Buffer offers robust performance and can be used for all PCR applications. It is a 2X master mix consisting of Phusion DNA Polymerase, deoxynucleotides and reaction buffer that has been optimized and includes MgCl 2. All that is required is the addition of template, primers and water.
